A novel planar polarity gene pepsinogen-like regulates wingless expression in a posttranscriptional manner.

摘要: Background: Planar cell polarity (PCP) originally referred to the coordination of global organ axes and individual within plane epithelium. More recently, it has been accepted that pertinent PCP regulators play essential roles not only in epithelial sheets, but also various rearranging cells. Results: We identified pepsinogen-like (pcl) as a new planar gene, using Drosophila wing epidermis model. Pcl protein is predicted belong family aspartic proteases. When pcl mutant clones were observed pupal wings, was disturbed both wild-type cells juxtaposed clone border. examined levels known proteins imaginal discs. The amount seven-pass transmembrane cadherin Flamingo (Fmi), one “core group” members, significantly decreased clones, whereas neither nor polarized localization Dachsous (Ds) at boundaries affected. In addition phenotype, mutation caused loss margins. Intriguingly, this most likely due dramatic decrease level Wingless (Wg) protein, wg transcripts. Conclusions: Our results raise possibility regulates Wg expression post-transcriptionally, PCP, by proteolytic cleavages. Developmental Dynamics 243:791–799, 2014. © 2014 Wiley Periodicals, Inc.