The JNK-like MAPK KGB-1 of Caenorhabditis elegans promotes reproduction, lifespan, and gene expressions for protein biosynthesis and germline homeostasis but interferes with hyperosmotic stress tolerance.

摘要: Aims: This study focused on the role of JNK-like MAPK (mitogen-activated protein kinase) KGB-1 (kinase, GLH-binding 1) for osmoprotection and other vital functions. Methods: We mapped expression patterns determined lifespan, reproduction survival rates as well changes in body volume, motility, GPDH (glycerol-3-phosphate dehydrogenase) activity glycerol production wildtype (WT), different signaling mutants (including a kgb-1 deletion mutant, kgb-1∆) RNAi-treated worms under control hyperosmotic conditions. KGB-1-mediated gene expressions were studied, instance, by RNA Sequencing, with resulting transcriptome data analyzed using orthology-based approaches. Results: Surprisingly, mutation/RNAi fos-1 (gene an AP-1, activator 1, element) significantly promoted resistance, even though was higher WT than kgb-1∆. moderate hyperosmolarity severe repressed kgb-1, fos-1, jun-1 another AP-1 expression. Transcriptome profiling revealed, down-regulated genes biosynthesis up-regulated membrane transporters kgb-1∆ GPDH-1 or detoxification WT, latter indicating cellular damage less effective WT. Conclusion: promotes lifespan fosters elements, biosynthesis, balanced gametogenesis, but inhibits perhaps order to energy consumption. Reduced biosyntheses enhanced transports most likely contribute high tolerance mutant easing burden existing chaperone machinery promoting regulatory volume increases upon stress.