Determination of Antithrombin

作者: EBERHARD F. MAMMEN

DOI: 10.1016/B978-0-12-077750-1.50011-8

关键词: Thrombin activityAntithrombinThrombinChromatographyActive stateEnzymeNeutralizationChemistry

摘要: Publisher Summary This chapter discusses the determination of antithrombin. One physiological functions antithrombin is inactivation thrombin activity. For activities in plasma or serum, it importance to consider difference between nonspecific and specific reactions. The reactions should be eliminated obtain more accurate results. Because readily adsorbed on glass surfaces, all determinations performed siliconized paraffinized glassware, plastic containers. With increasing temperatures, rate amount neutralized can become greater. enzymatic nature antithrombin, concentration only decrease progressively. Therefore, methods consist a series measurements at different time intervals. neutralization varies with both When high relative concentration, facilitated slowly some always remain an active state.

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