Selective formation of mannosyl-l-arabitol lipid by Pseudozyma tsukubaensis JCM16987
作者： Tomotake Morita , Tokuma Fukuoka , Ayana Kosaka , Tomohiro Imura , Hideki Sakai
DOI: 10.1007/S00253-015-6575-4
关键词: Glycolipid 、 Arabitol 、 Acid hydrolysis 、 Sugar alcohol 、 Critical micelle concentration 、 Mannose 、 Chromatography 、 Sugar 、 Chemistry 、 Diastereomer
摘要: To develop a structural homolog of mannosylerythritol lipids (MELs), Pseudozyma tsukubaensis JCM16987 (known to be specific producer the diastereomer type mono-acetylated MEL (MEL-B)) was cultivated in medium containing 4 % (w/v) olive oil as primary carbon source and L-arabitol supplemental sugar alcohol. Based on thin-layer chromatography (TLC), glycolipid extract showed two major spots corresponding MEL-B an unknown (GL1). high-performance liquid after acid hydrolysis, GL1 from culture peaks identical mannose arabitol using analysis column, one peak detected chiral resolution column. NMR analysis, identified mannosyl-L-arabitol lipid (MLAL-B) consisting mannose, with moiety. The observed critical micelle concentration (CMC) surface tension at CMC (γCMC) MLAL-B were 1.2 × 10(-5) M 32.8 mN/m, which significantly higher than (CMC = 3.1 × 10(-6) γcmc = 26.1 mN/m). Furthermore, based water-penetration scan, efficiently formed lamellar phase (Lα) myelins broad range. Thus, present hydrophilicity and/or water solubility increased our understanding environmentally advanced biosurfactants.
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