Second-harmonic generation in collagen as a potential cancer diagnostic parameter.

摘要: The fibrillar collagen network in tumor and normal tissues is different due to remodeling of the extracellular matrix during malignant process. Collagen type I fibers have crystalline noncentrosymmetric properties required for generating second-harmonic signal. content structure were studied by imaging generation (SHG) signal frozen sections from three tissues, osteosarcoma, breast carcinoma, melanoma, compared with corresponding bone/femur, breast, dermis/skin. density was measured as percentage pixels containing SHG tissue images, material parameters such second-order nonlinear optical susceptibility given d22 coefficient an empirical anisotropy parameter used characterize structure. Generally, had much more than tissues. In a cap seen at periphery, further into tumors, distribution sparse heterogeneous. difference reflected two times higher lower values Together, differences content, distribution, show that signature promising diagnostic marker.