Allergy to bovine beta-lactoglobulin: specificity of human IgE to tryptic peptides.

摘要: Background Bovine β-lactoglobulin (Blg) is a major cow's milk allergen. It the main whey protein, without any counterpart in human milk. Blg chemical hydrolysates appeared to retain most of immunoreactivity native protein. Allergenicity has already been shown be associated with four peptides derived from cyanogen bromide cleavage Blg. Objectives To map allergenic epitopes (e.g. regions molecule able bind IgE) on using specific IgE sera 46 milk-allergic patients as probe. Methods Direct and competitive inhibition enzyme immunoassays involving immobilized protein or purified tryptic cleavage. Results Several capable specifically binding IgEs were identified classified according intensity frequency responses. The fragments (41–60), (102–124) (149–162) recognized by 92, 97 89% sera, respectively, whilst second group which contained (1–8) (25–40) was 58 72% population. A third group, comprising (9–14), (84–91) (92–100), still detected more than 40% sera. Conclusion Three epitopes, large majority antibodies. Numerous other are scattered all along sequence.