In vitro model of platelet-endothelial activation due to cigarette smoke under cardiovascular circulation conditions.
作者： Gaurav Girdhar , Sulan Xu , Jolyon Jesty , Danny Bluestein
DOI: 10.1007/S10439-008-9503-2
关键词: Hemodynamics 、 Platelet 、 Inflammation 、 Endocrinology 、 Endothelial activation 、 Endothelial stem cell 、 Internal medicine 、 Nicotine 、 Platelet activation 、 Biochemistry 、 In vitro 、 Chemistry
摘要: Cigarette smoke has been shown to increase platelet activation and endothelial cell (EC) adhesion molecule expression. In the present study, we utilized a hemodynamic shearing device (HSD) investigate above effects in vitro combined system of platelets cultured HUVECs (Human Umblical Vein ECs) under physiological shear stress. We investigated alteration E-selectin expression on ECs upon exposure to: (1) nicotine-free extract (NFE), (2) alone, (3) NFE additionally confirmed protective effect nicotine activation. found that: (i) surface was significantly increased simultaneous relative either or alone (p < 0.05). (ii) Platelet presence (iii) Nicotine (200 nM) when added NFE, reduced due 0.05), an by conventional cigarette extracts which contain therefore conclude (a) additively EC expression, (b) modulates regardless ECs.
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