Brain ankyrin. A membrane-associated protein with binding sites for spectrin, tubulin, and the cytoplasmic domain of the erythrocyte anion channel.

作者: J Q Davis , V Bennett

DOI: 10.1016/S0021-9258(18)90728-3

关键词: TubulinBiochemistryANK1BiophysicsEPB41BiologyMembrane proteinAnkyrin bindingSpectrin bindingSpectrinAnkyrin

摘要: Brain ankyrin was purified from pig brain membranes in milligram quantities by a procedure involving affinity chromatography on erythrocyte spectrinagarose. included two polypeptides of Mr = 210,000 and 220,000 that were nearly identical peptide mapping monomers solution. are closely related proteins with the following properties common: 1) shared antigenic sites, 2) high-affinity binding to spectrin beta subunit at midregion tetramers, 3) site for cytoplasmic domain anion channel, 4) tubulin, 5) similar structure protease-resistant 72,000 contains spectrin-binding activity domains 95,000 (brain ankyrin) or 90,000 (erythrocyte contain sites both tubulin channel. is present about 100 pmol/mg membrane protein demyelinated based radioimmunoassay antibody raised against ankyrin-agarose. tetramers 30 protein. thus sufficient amounts attach membranes. also may microtubules independently has potential interconnect spectrin-associated actin filaments.

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