Transcription mapping of a 100 kb locus of Plasmodium falciparum identifies an intergenic region in which transcription terminates and reinitiates.

摘要: We have mapped Plasmodium falciparum erythrocytic stage transcription units on chromosome 10 in the vicinity of gene encoding glycophorin binding protein (GBP130) using yeast artificial chromosomes (YACs). Three are clustered a 40 kb region. Two these genes closely linked, separated by less than 2 kb. Nuclear run-on data demonstrate that two genes, though unidirectional, is monocistronic. Within this intergenic region sites at which upstream terminates and GBP130 initiates. These studies represent first description minimal necessary cis-acting elements for termination initiation protozoan parasite.