Cholinergic innervation of the rat hippocampus as revealed by choline acetyltransferase immunocytochemistry: A combined light and electron microscopic study
作者： Michael Frotscher , Csaba Léránth
关键词: Neuroscience 、 Hippocampus proper 、 Choline acetyltransferase 、 Postsynaptic potential 、 Immunocytochemistry 、 Biology 、 Fascia dentata 、 Biophysics 、 Cholinergic Fibers 、 Cholinergic 、 Hippocampal formation
摘要: The cholinergic innervation of the rat hippocampus proper and fascia dentata was investigated by using a monoclonal antibody against choline acetyltransferase (ChAT). At light microscopic level, thin varicose ChAT-immunoreactive fibers were observed mainly in vicinity pyramidal granular layers where they formed fine network around proximal dendrites granule cells. In addition, many ChAT-immuno-reactive found hilar region stratum oriens, radiatum, lacunosum-moleculare all hippocampal sectors. Electron analysis revealed ChAT immunoreactivity unmyelinated axons terminals which established synaptic contacts. Asymmetric contacts on small spines dendritic molecular layer dentata. Symmetric cell bodies Both symmetric asymmetric occurred shafts. serial sections, allows identification postsynaptic elements, suggests that cells, nonpyramidal neurons receive input.
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