Site- and strand-specific nicking in vitro at oriT by the traY-traI endonuclease of plasmid R100
作者： S. Inamoto , Y. Yoshioka , E. Ohtsubo
DOI: 10.1016/S0021-9258(18)99193-3
关键词: DNA 、 Nicking enzyme 、 Molecular biology 、 A-site 、 Plasmid 、 Endonuclease 、 Genetic transfer 、 Gene 、 Base pair 、 Biology
摘要: Abstract We developed an in vitro system to reproduce a site- and strand-specific nicking at the oriT region of plasmid R100. The reaction was dependent on purified TraY protein lysate, which prepared from cells overproducing TraI protein. This supports idea that products two genes, traY traI, constitute endonuclease introduces specific nick vivo conjugative plasmids related were "complex" DNA molecules with covalently linked 5'-end nick. introduced strand, is supposed be transferred recipient during conjugation, located site 59 base pairs upstream binding site, sbyA.
sci-hub.st 参考文章(36)
A Sancar, A M Hack, W D Rupp, Simple method for identification of plasmid-coded proteins. Journal of Bacteriology. ,vol. 137, pp. 692- 693 ,(1979) , 10.1128/JB.137.1.692-693.1979
S Inamoto, E Ohtsubo, Specific binding of the TraY protein to oriT and the promoter region for the traY gene of plasmid R100. Journal of Biological Chemistry. ,vol. 265, pp. 6461- 6466 ,(1990) , 10.1016/S0021-9258(19)39349-4
W B Dempsey, Integration host factor and conjugative transfer of the antibiotic resistance plasmid R100. Journal of Bacteriology. ,vol. 169, pp. 4391- 4392 ,(1987) , 10.1128/JB.169.9.4391-4392.1987
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
W Pansegrau, G Ziegelin, E Lanka, Covalent association of the traI gene product of plasmid RP4 with the 5'-terminal nucleotide at the relaxation nick site. Journal of Biological Chemistry. ,vol. 265, pp. 10637- 10644 ,(1990) , 10.1016/S0021-9258(18)86994-0
Mahmoud Abdel-Monem, Hans-Frieder Lauppe, Jürgen Kartenbeck, Hildegard Dürwald, Hartmut Hoffmann-Berling, Enzymatic unwinding of DNA. III. Mode of action of Escherichia coli DNA unwinding enzyme. Journal of Molecular Biology. ,vol. 110, pp. 667- 685 ,(1977) , 10.1016/S0022-2836(77)80083-1
Joachim Messing, New M13 vectors for cloning. Methods in Enzymology. ,vol. 101, pp. 20- 78 ,(1983) , 10.1016/0076-6879(83)01005-8
R.E. Depew, L.F. Liu, J.C. Wang, Interaction between DNA and Escherichia coli protein omega. Formation of a complex between single-stranded DNA and omega protein. Journal of Biological Chemistry. ,vol. 253, pp. 511- 518 ,(1978) , 10.1016/S0021-9258(17)38239-X
S Inamoto, Y Yoshioka, E Ohtsubo, Identification and characterization of the products from the traJ and traY genes of plasmid R100. Journal of Bacteriology. ,vol. 170, pp. 2749- 2757 ,(1988) , 10.1128/JB.170.6.2749-2757.1988
S A McIntire, W B Dempsey, oriT sequence of the antibiotic resistance plasmid R100. Journal of Bacteriology. ,vol. 169, pp. 3829- 3832 ,(1987) , 10.1128/JB.169.8.3829-3832.1987