Expression of the Evi-1 zinc finger gene in 32Dc13 myeloid cells blocks granulocytic differentiation in response to granulocyte colony-stimulating factor.

摘要: Expression of the Evi-1 gene is frequently activated in murine myeloid leukemias by retroviral insertions immediately 5' or 90 kb gene. The product a nuclear, DNA-binding zinc finger protein 145 kDa. On basis properties cell lines which activated, it has been hypothesized that its expression blocks normal differentiation. To explore this proposed role, we have constructed retrovirus vector containing and examined effects on an interleukin-3-dependent line differentiates response to granulocyte colony-stimulating factor (G-CSF). these cells did not alter growth requirements cells. However, blocked ability express myeloperoxidase terminally differentiate granulocytes G-CSF. This effect was due altered G-CSF receptor changes initial responses These results support hypothesis inappropriate interferes with differentiate.