Lipopolysaccharide from Prevotella nigrescens stimulates osteoclastogenesis in cocultures of bone marrow mononuclear cells and primary osteoblasts.

摘要: Background and objective Lipopolysaccharide is thought to be a major virulence factor of pathogens associated with periodontal diseases believed stimulate bone resorption in vivo. Although Prevotella nigrescens has been implicated periodontitis, its role osteoclastogenesis not reported. In this study, we investigated the effects lipopolysaccharide from P. on formation osteoclasts production cytokines related osteoclast differentiation. Material methods Mouse marrow mononuclear cells were cultured presence macrophage colony-stimulating (M-CSF) receptor activator nuclear kappaB ligand (RANKL), or without lipopolysaccharide. Bone also cocultured calvarial osteoblastic absence Osteoclast was determined by tartrate-resistant acid phosphatase cytochemistry. The osteoprotegerin (OPG), M-CSF, tumor necrosis alpha (TNF-alpha), transforming growth factor-beta (TGF-beta) prostaglandin E2 (PGE2) enzyme-linked immunosorbent assay (ELISA). Results inhibited differentiation M-CSF RANKL. However, coculture system, stimulated osteoclastogenesis. Notably, decreased OPG but increased TGF-beta secretion. addition, treatment PGE2 during late stage culture period. There no difference TNF-alpha production. Conclusion These results demonstrate that stimulates system decreasing increasing PGE2. Through mechanisms involving these factors, may cause alveolar diseases.