Maternal Intake of n-3 Polyunsaturated Fatty Acids During Pregnancy Is Associated With Differential Methylation Profiles in Cord Blood White Cells

摘要: A healthy diet during pregnancy is pivotal for the offspring health at birth and later in life. N-3 polyunsaturated fatty acids (n-3 PUFAs) are not endogenously produced humans exclusively derived from diet. They fetus growth neuronal development seem beneficial reducing risk of cardiometabolic diseases preventing allergic disorders by modulating inflammatory immune response. In present study, we investigated association between maternal intakes n-3PUFAs, profiled on erythrocyte membranes term, DNA methylation cord blood mononuclear cells a sample 118 mother-newborn pairs randomly drawn "Feeding fetus' low-grade inflammation insulin-resistance" study cohort. PUFA content validated biomarker to measure objectively medium term intake n-3 PUFAs. Based distribution whole cohort mothers, identified mothers with low concentration 75th percentile). The HumanMethylation450 BeadChip (Illumina) was used epigenome-wide using Infinium Methylation Assay. overall level different three groups while there significant difference levels certain sites. Indeed, 8,503 sites had significantly methylations high groups, 12,716 18,148 groups. We found differentially methylated genes that belong prevalently pathways signal transduction, metabolism, downstream signaling G protein-coupled receptors, gene expression. Within these pathways, four genes, namely, MSTN, IFNA13, ATP8B3, GABBR2, involved onset insulin resistance adiposity, innate response, phospholipid translocation across cell membranes, mechanisms addiction fat diet, alcohol, sweet taste. conclusion, findings this preliminary investigation suggest PUFAs has potential influence methylation. Validation results larger biological significance impact phenotype warranted.