Impact of the antifungal protein PgAFP from Penicillium chrysogenum on the protein profile in Aspergillus flavus

摘要: Antifungal proteins produced by molds are generally small, highly basic, and cysteine-rich. The best known effects of these include morphological changes, metabolic inactivation, membrane perturbation on sensitive fungi. Reactive oxygen species (ROS) generation leads to apoptosis, with G -protein playing a key role in transduction cell death signals. antifungal protein PgAFP from Penicillium chrysogenum inhibits growth some toxigenic molds. Here we analyzed the effect Aspergillus flavus. For this, comparative proteomic analysis was used identify whole profile change abundance after treatment. provoked changes related reduced energy metabolism, wall integrity alteration, increased stress response due higher levels ROS. observed abundance, favoring glutathione concentration as well heat shock proteins, do not seem be enough avoid necrosis. decreased chitin deposition PgAFP-treated A. flavus is attributed lower relative quantity Rho1. β subunit seems underlying reason for modulation apoptosis hyphae. We propose Rho1 CpcB main factors mode action Additionally, enzymes essential biosynthesis aflatoxin were no longer detectable hyphae at 24 h, following treatment PgAFP. This presents promising PgAFP, which may prevent producing mycotoxins. However, impact actual production requires further study.