Improved two-step PCR method
作者： Satoshi Orita , Yuko Aono , Hisanaga Igarashi , Akihiko Sato
DOI:
关键词: Multiplex polymerase chain reaction 、 Primer (molecular biology) 、 Molecular biology 、 Chemistry 、 Primer dimer 、 Polymerase chain reaction optimization 、 Pcr method 、 Two step 、 Polymerase chain reaction
摘要: The present invention relates to a two-step polymerase chain reaction method with high sensitivity and specificity, wherein the primer concentration in first-step PCR is very low.
lens.org 参考文章(4)
Thierry Livache, Robert Teoule, Brigitte Fouque, Sylvie Sauvaigo, Method for detecting specific nucleic acid sequences and applications of same. ,(1990)
Henry A. Erlich, PCR Technology: Principles and Applications for DNA Amplification ,(1989)
Stanley Robert Bouma, Thomas G. Laffler, Detection and amplification of target nucleic acid sequences ,(1989)
D. J. Kemp, D. B. Smith, S. J. Foote, N. Samaras, M. G. Peterson, Colorimetric detection of specific DNA segments amplified by polymerase chain reactions Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 2423- 2427 ,(1989) , 10.1073/PNAS.86.7.2423