Method for mapping a nucleic acid

摘要: A method for observing and determining the size of individual molecules weight distribution a sample containing varying size, which involves placing deformable or nondeformable molecule in medium, subjecting to an external force, thereby causing conformational and/or positional changes, then measuring these changes. Preferred ways measure changes include: (1) rate at returns relaxed state after termination (2) becomes oriented new direction when perturbing force is changed, (3) rotates, (4) length molecule, particularly it least partially stretched, (5) one diameter spherical ellipsoidal molecule. Measurements relaxation, reorientation, rotation rates, as well can be made using light microscope connected image processor. Molecule reorientation also determined combined with spectroscopic device. The invention useful polymer molecules, such nucleic acids, used determine map location restriction digests. Breakage large mounted on slide prevented by condensing before mounting unfolding they have been placed matrix.