Dentin Sialophosphoprotein–derived Proteins in the Dental Pulp
作者： R. Yamamoto , S. Oida , Y. Yamakoshi
关键词: Dentin phosphoprotein 、 Dentin sialoprotein 、 Odontoblast 、 Dentin sialophosphoprotein 、 Molecular biology 、 Glycoprotein 、 Messenger RNA 、 Gel electrophoresis 、 Chemistry 、 Dentin
摘要: Porcine dentin sialophosphoprotein (DSPP), the most abundant noncollagenous protein in dentin, is critical for proper mineralization of tooth dentin. DSPP processed by proteases into 3 major domains: sialoprotein (DSP), glycoprotein (DGP), and phosphoprotein (DPP). There are at least 2 mRNA variants expressed from Dspp gene: one encodes full-length (DSP+DGP+DPP); other only DSP. The shorter transcript generated through use a polyadenylation signal within intron 4, immediately following DSP coding region (DGP DPP encoded exon 5). We fractionated DSPP-derived proteins dental pulp developing porcine incisors using heparin chromatography. was identified, but little could be detected any fractions. BMP-1 digestion extracted did not generate new bands on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (indicating an absence intact DSPP), although the...
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