The Recombinant Human TRPV6 Channel Functions as Ca2+Sensor in Human Embryonic Kidney and Rat Basophilic Leukemia Cells
作者： Matthias Bödding , Ulrich Wissenbach , Veit Flockerzi
关键词: Intracellular 、 Reversal potential 、 BAPTA 、 Endoplasmic reticulum 、 EGTA 、 Biology 、 Thapsigargin 、 Cell culture 、 Biochemistry 、 Cell biology 、 Transient receptor potential channel
摘要: The activation mechanism of the recently cloned human transient receptor potential vanilloid type 6 (TRPV6) channel, originally termed Ca2+ transporter-like protein and transporter 1, was investigated in whole-cell patch-clamp experiments using transiently transfected embryonic kidney rat basophilic leukemia cells. TRPV6-mediated currents are highly Ca2+-selective, show a strong inward rectification, reverse at positive potentials, which is similar to store-operated entry electrically nonexcitable gating TRPV6 channels strongly dependent on cytosolic free concentration; lowering intracellular concentration results influx, current amplitude correlates with EGTA or BAPTA concentration. This also case for absence extracellular divalent cations; compared endogenous nontransfected cells, these monovalent reveal differences reversal potential, slope very negative potentials. Release stored Ca2+by inositol 1,4,5-trisphosphate and/or sarco/endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin appears not be involved channel both cell lines but, readily activates release-activated current. In conclusion, TRPV6, expressed cells functions as Ca2+-sensing independently procedures known deplete stores.
sci-hub.se 参考文章(34)
X.-M. Xia, B. Fakler, A. Rivard, G. Wayman, T. Johnson-Pais, J. E. Keen, T. Ishii, B. Hirschberg, C. T. Bond, S. Lutsenko, J. Maylie, J. P. Adelman, Mechanism of calcium gating in small-conductance calcium-activated potassium channels Nature. ,vol. 395, pp. 503- 507 ,(1998) , 10.1038/26758
Monica J. S. Nadler, Meredith C. Hermosura, Kazunori Inabe, Anne-Laure Perraud, Qiqin Zhu, Alexander J. Stokes, Tomohiro Kurosaki, Jean-Pierre Kinet, Reinhold Penner, Andrew M. Scharenberg, Andrea Fleig, LTRPC7 is a Mg·ATP-regulated divalent cation channel required for cell viability Nature. ,vol. 411, pp. 590- 595 ,(2001) , 10.1038/35079092
Michael J. Berridge, Peter Lipp, Martin D. Bootman, The versatility and universality of calcium signalling Nature Reviews Molecular Cell Biology. ,vol. 1, pp. 11- 21 ,(2000) , 10.1038/35036035
Lixia Yue, Ji-Bin Peng, Matthias A. Hediger, David E. Clapham, CaT1 manifests the pore properties of the calcium-release-activated calcium channel Nature. ,vol. 410, pp. 705- 709 ,(2001) , 10.1038/35070596
Ulrich Wissenbach, Barbara A. Niemeyer, Thomas Fixemer, Arne Schneidewind, Claudia Trost, Adolfo Cavalié, Katrin Reus, Eckart Meese, Helmut Bonkhoff, Veit Flockerzi, Expression of CaT-like, a Novel Calcium-selective Channel, Correlates with the Malignancy of Prostate Cancer Journal of Biological Chemistry. ,vol. 276, pp. 19461- 19468 ,(2001) , 10.1074/JBC.M009895200
C. E. Stansfeld, J. Roper, J. Ludwig, R. M. Weseloh, S. J. Marsh, D. A. Brown, O. Pongs, Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-à-go-go channels in a stably transfected cell line Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 9910- 9914 ,(1996) , 10.1073/PNAS.93.18.9910
D Müller, JGJ Hoenderop, IC Meij, LPJ Van den Heuvel, NVAM Knoers, AI Den Hollander, P Eggert, V Garcı&́a-Nieto, F Claverie-Martı&́n, RJM Bindels, None, Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1). Genomics. ,vol. 67, pp. 48- 53 ,(2000) , 10.1006/GENO.2000.6203
Adam Zweifach, Richard S. Lewis, Slow Calcium-dependent Inactivation of Depletion-activated Calcium Current. STORE-DEPENDENT AND -INDEPENDENT MECHANISMS Journal of Biological Chemistry. ,vol. 270, pp. 14445- 14451 ,(1995) , 10.1074/JBC.270.24.14445
Leonardo Fierro, Per-Eric Lund, Anant B. Parekh, Comparison of the activation of the Ca2+ release-activated Ca2+ current ICRAC to InsP3 in Jurkat T-lymphocytes, pulmonary artery endothelia and RBL-1 cells. Pflügers Archiv: European Journal of Physiology. ,vol. 440, pp. 580- 587 ,(2000) , 10.1007/S004240000336
Robin M. Bush, Predicting adaptive evolution. Nature Reviews Genetics. ,vol. 2, pp. 387- 392 ,(2001) , 10.1038/35072023