Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates
作者： J. E. Gee , B. K. De , P. N. Levett , A. M. Whitney , R. T. Novak
DOI: 10.1128/JCM.42.8.3649-3654.2004
关键词: Microbiology 、 Gene duplication 、 Bacterial genetics 、 Bacteria 、 Ribosomal RNA 、 Ribosomal DNA 、 Brucella 、 16S ribosomal RNA 、 Genetics 、 Gene 、 Biology
摘要: Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans animals. Current identification methods rely on biochemical tests that may require up to 7 days results. We sequenced 16S rRNA genes 65 strains along with 17 related likely present differential diagnostic challenge. All gene sequences were determined be identical clearly different from strains, suggesting sequencing is reliable tool rapid genus-level spp. their differentiation closely organisms.
sci-hub.se 参考文章(42)
Dannie G. Hollis, Robert E. Weaver, William A. Clark, Perry. Riley, Identification of unusual pathogenic gram-negative aerobic and facultatively anaerobic bacteria Williams & Wilkins. ,(1985)
B J Bricker, S M Halling, Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. Journal of Clinical Microbiology. ,vol. 33, pp. 1640- 1642 ,(1995) , 10.1128/JCM.33.6.1640-1642.1995
Gaafar Mohammed Malik, A clinical study of brucellosis in adults in the Asir region of southern Saudi Arabia. American Journal of Tropical Medicine and Hygiene. ,vol. 56, pp. 375- 377 ,(1997) , 10.4269/AJTMH.1997.56.375
E Moreno, E Stackebrandt, M Dorsch, J Wolters, M Busch, H Mayer, Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria. Journal of Bacteriology. ,vol. 172, pp. 3569- 3576 ,(1990) , 10.1128/JB.172.7.3569-3576.1990
C.D. MILLER, J.R. SONGER, J.F. SULLIVAN, A Twenty-Five Year Review of Laboratory-Acquired Human Infections at the National Animal Disease Center American Industrial Hygiene Association Journal. ,vol. 48, pp. 271- 275 ,(1987) , 10.1080/15298668791384733
M. Da Costa, J.-P. Guillou, B. Garin-Bastuji, M. Thiébaud, G. Dubray, Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification Journal of Applied Microbiology. ,vol. 81, pp. 267- 275 ,(1996) , 10.1111/J.1365-2672.1996.TB04328.X
Shahzi S Iqbal, Michael W Mayo, John G Bruno, Burt V Bronk, Carl A Batt, James P Chambers, A review of molecular recognition technologies for detection of biological threat agents. Biosensors and Bioelectronics. ,vol. 15, pp. 549- 578 ,(2000) , 10.1016/S0956-5663(00)00108-1
Ronald A. Greenfield, Douglas A. Drevets, Linda J. Machado, Gene W. Voskuhl, Paul Cornea, Michael S. Bronze, Bacterial Pathogens as Biological Weapons and Agents of Bioterrorism The American Journal of the Medical Sciences. ,vol. 323, pp. 299- 315 ,(2002) , 10.1097/00000441-200206000-00003
Hürrem Bodur, Neriman Balaban, Sebahat Aksaray, Vedat Yetener, Esragül Akinci, Aylin Çolpan, Ayse Erbay, Biotypes and antimicrobial susceptibilities of Brucella isolates. Scandinavian Journal of Infectious Diseases. ,vol. 35, pp. 337- 338 ,(2003) , 10.1080/00365540310008348
Betsy J. Bricker, PCR as a diagnostic tool for brucellosis Veterinary Microbiology. ,vol. 90, pp. 435- 446 ,(2002) , 10.1016/S0378-1135(02)00228-6