Expert consensus on an in vitro approach to assess pulmonary fibrogenic potential of aerosolized nanomaterials

摘要: The increasing use of multi-walled carbon nanotubes (MWCNTs) in consumer products and their potential to induce adverse lung effects following inhalation has lead much interest better understanding the hazard associated with these nanomaterials (NMs). While current regulatory requirement for substances concern, such as MWCNTs, many jurisdictions is a 90-day rodent test, monetary, ethical, scientific concerns this test led an international expert group convene Washington, DC, USA, discuss alternative approaches evaluate toxicity MWCNTs. Pulmonary fibrosis was identified key outcome linked MWCNT exposure, recommendations were made on design vitro assay that predictive fibrotic takes weeks or months develop vivo, system may more rapidly predict fibrogenic by monitoring pro-fibrotic mediators (e.g., cytokines growth factors). Therefore, workshop discussions focused necessary specifications related development evaluation system. Recommendations designing using lung-relevant cells co-cultured at air–liquid interface assess pro-fibrogenic aerosolized while considering human-relevant dosimetry NM life cycle transformations. provided fundamental components will be subsequently expanded testing strategy pulmonary generate data enable effective risk assessment NMs.