Immunization via hair follicles by topical application of naked DNA to normal skin.
作者： Hongran Fan , Qun Lin , Georgiann R. Morrissey , Paul A. Khavari
DOI: 10.1038/12856
关键词: HBsAg 、 Genetic transfer 、 Naked DNA 、 Biology 、 Virology 、 Hair follicle 、 Recombinant DNA 、 Immune system 、 Plasmid 、 Expression vector
摘要: In order to test the immune response generated small amounts of foreign protein in skin, we applied naked DNA aqueous solution untreated normal skin. Topical application plasmid expression vectors for lacZ and hepatitis B surface antigen (HBsAg) intact skin induced antigen-specific responses that displayed TH2 features. For HBsAg, specific antibody cellular were same magnitude as those produced by intramuscular injection commercially available recombinant HBsAg polypeptide vaccine. Finally, topical gene transfer was dependent on presence hair follicles.
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sci-hub.se 参考文章(18)
H Grey, G Ishioka, F V Chisari, J Fikes, A Sette, C Crimi, B D Livingston, R W Chesnut, The hepatitis B virus-specific CTL responses induced in humans by lipopeptide vaccination are comparable to those elicited by acute viral infection. Journal of Immunology. ,vol. 159, pp. 1383- 1392 ,(1997)
Clayton W. Beard, Peter W. Mason, Out on the farm with DNA vaccines Nature Biotechnology. ,vol. 16, pp. 1325- 1328 ,(1998) , 10.1038/4298
Andrea C. Lauer, Linda M. Lieb, C. Ramachandran, Gordon L. Flynn, Norman D. Weiner, Transfollicular Drug Delivery Pharmaceutical Research. ,vol. 12, pp. 179- 186 ,(1995) , 10.1023/A:1016250422596
De-chu Tang, Zhongkai Shi, David, T Curiel, Vaccination onto bare skin Nature. ,vol. 388, pp. 729- 730 ,(1997) , 10.1038/41917
C. S. Seitz, Q. Lin, H. Deng, P. A. Khavari, Alterations in NF-κB function in transgenic epithelial tissue demonstrate a growth inhibitory role for NF-κB Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 2307- 2312 ,(1998) , 10.1073/PNAS.95.5.2307
Bo Lu, Howard J. Federoff, Yibin Wang, Lowell A. Goldsmith, Glynis Scott, Topical Application of Viral Vectors for Epidermal Gene Transfer Journal of Investigative Dermatology. ,vol. 108, pp. 803- 808 ,(1997) , 10.1111/1523-1747.EP12292254
Jeffrey J. Berti, James J. Lipsky, Transcutaneous Drug Delivery: A Practical Review Mayo Clinic Proceedings. ,vol. 70, pp. 581- 586 ,(1995) , 10.4065/70.6.581
M Liu, Immunization of non-human primates with DNA vaccines Vaccine. ,vol. 15, pp. 909- 912 ,(1997) , 10.1016/S0264-410X(96)00280-0
Thomas Tüting, Walter J. Storkus, Louis D. Falo, DNA immunization targeting the skin : Molecular control of adaptive immunity Journal of Investigative Dermatology. ,vol. 111, pp. 183- 188 ,(1998) , 10.1046/J.1523-1747.1998.00261.X
J. M. Janda, S. L. Abbott, R. A. Brenden, Overview of the etiology of wound infections with particular emphasis on community-acquired illnesses. European Journal of Clinical Microbiology & Infectious Diseases. ,vol. 16, pp. 189- 201 ,(1997) , 10.1007/BF01709581