Enhanced salt sensitivity following shRNA silencing of neuronal TRPV1 in rat spinal cord

摘要: To investigate the effects of selective knockdown TRPV1 channels in lower thoracic and upper lumbar segments spinal cord, dorsal root ganglia (DRG) mesenteric arteries on rat blood pressure responses to high salt intake. short-hairpin RNA (shRNA) was delivered using intrathecal injection (6 μg·kg−1·d−1, for 3 d). Levels tyrosine hydroxylase expression were determined by Western blot analysis. Systolic mean arterial (MAP) examined tail-cuff direct measurement, respectively. In rats injected with control shRNA, high-salt diet (HS) caused higher systolic compared normal-salt (NS) (HS:149±4 mmHg; NS:126±2 mmHg, P<0.05). Intrathecal shRNA significantly increased both HS NS (HS:169±3 NS:139±2 mmHg). The increases greater than (HS: 13.9%±1.8%; NS: 9.8±0.7, After treatment, horn DRG T8-L3 knocked down a extent rats. Blockade α1-adrenoceptors abolished shRNA-induced pressor effects. level Selective DRG, enhanced prohypertensive intake, suggesting that these sites protect against sensitivity, possibly via suppression sympatho-excitatory responses.