Transport of sugars in yeasts. II. Mechanisms of utilization of disaccharides and related glycosides.

摘要: Abstract 1. The outstanding ability of some yeasts for the fermentation certain disaccharides and recent identification specific transport systems uptake hexoses prompted investigation likely involvement processes in oligosaccharides. 2. kinetics specificity main types glycosides by intact their hydrolysis cell-free extracts have been studied. 3. Extracellular trapping can interfere with β-fructosides S. cerevisiae that α-galactosides carlsbergensis . This observation added to fact pH-activity curve substrate are same hydrolysis, demonstrates first step sucrose, melibiose, analogues is a outside membrane, followed liberated hexoses. 4. On contrary, utilization maltose lactose fragilis show curves markedly different vivo vitro Maltose be faster than constituent was not affected addition system extracellular combined evidence strongly suggests lactose, splitting intracellular enzymes.