Genetic variability and molecular responses of root penetration in cotton

作者: Natalya Y. Klueva , Rama C. Joshi , Chandrashekhar P. Joshi , David B. Wester , Richard E. Zartman

DOI: 10.1016/S0168-9452(00)00205-3

关键词: Genetic variationBiologyGenetic variabilityDifferential displayMalvaceaeRoot systemHorticultureFiber cropSoil horizonPenetration (firestop)Botany

摘要: Abstract Compacted soils restrict root penetration hindering productivity. In this paper, genetic variability of cotton (Gossipium spp.) capacity to penetrate hard soil layers and the patterns gene expression during event were investigated. To mimic layers, wax–petrolatum mixtures used. Genetic among 27 genotypes for disks 500–700 g wax/kg mixture was high indicating that breeding efforts targeted improve trait can be successful. tips a strain with penetrating ability (G. hirsutum HS 200) which penetrated through (P), quantity four polypeptides molecular weights 35–66 kDa increased compared those grew in absence mechanical impedance (NP). Differential display showed significant differences sets mRNA expressed P NP roots. Out total 917 cDNAs scored differential experiment, 118 cDNAs, or 13%, specific roots hence could associated event. Further detailed study will pinpoint factors involved cotton.

参考文章(22)
Ying-Fei Huang, Wayne R. Jordan, Rod A. Wing, Page W. Morgan, Gene expression induced by physical impedance in maize roots Plant Molecular Biology. ,vol. 37, pp. 921- 930 ,(1998) , 10.1023/A:1006034411529
F. W. G. Baker, Drought resistance in cereals Drought resistance in cereals.. ,(1989)
Ping Song, Etsuo Yamamoto, Randy D. Allen, Improved procedure for differential display of transcripts from cotton tissues Plant Molecular Biology Reporter. ,vol. 13, pp. 174- 181 ,(1995) , 10.1007/BF02668789
Jürgen Logemann, Jeff Schell, Lothar Willmitzer, Improved method for the isolation of RNA from plant tissues. Analytical Biochemistry. ,vol. 163, pp. 16- 20 ,(1987) , 10.1016/0003-2697(87)90086-8
M. J. Kasperbauer, W. J. Busscher, Genotypic Differences in Cotton Root Penetration of a Compacted Subsoil Layer Crop Science. ,vol. 31, pp. 1376- 1378 ,(1991) , 10.2135/CROPSCI1991.0011183X003100050063X
D. C. Malcolm, E. W. Carson, The Plant Root and Its Environment. The Journal of Ecology. ,vol. 63, pp. 715- ,(1975) , 10.2307/2258747
C. P. Joshi, N. Y. Klueva, K. J. Morrow, H. T. Nguyen, Expression of a unique plastid-localized heat-shock protein is genetically linked to acquired thermotolerance in wheat Theoretical and Applied Genetics. ,vol. 95, pp. 834- 841 ,(1997) , 10.1007/S001220050633
Howard M. Taylor, Herbert R. Gardner, Use of wax substrates in root penetration studies. Soil Science Society of America Journal. ,vol. 24, pp. 79- 81 ,(1960) , 10.2136/SSSAJ1960.03615995002400020004X