Keratin-tannic acid complex nanoparticles as pH/GSH dual responsive drug carriers for doxorubicin.
作者： Jiang Yuan , Jian Shen , Jinsong Du , Jie Dou , Xiao Han
DOI: 10.1080/09205063.2021.1906074
关键词: Nanoparticle 、 Doxorubicin 、 Glutathione 、 Biophysics 、 Tannic acid 、 Chemistry 、 Drug carrier 、 Keratin 、 A549 cell 、 Tumor microenvironment
摘要: Drug-loaded nanoparticles have been widely used in the field of tumor treatment due to their low side effects and reduced frequency administration. In this study, pH glutathione (GSH) dual-responsive keratin-tannic acid (TA) complex were established trigger drug release under microenvironments. Reductive keratin was first extracted using a reduction method. Then, keratin-TA (KNPs) self-assembled via non-covalent interaction further stabilized by self-crosslinking thiols. This method facile green without chemicals during whole procedure. KNPs exhibited GSH dual responsiveness as well charge reversibility simulated microenvironment. The anticancer doxorubicin (DOX) loaded on hydrophobicity hydrogen bonds. accelerated mimicked microenvironments, performing high toxic against A549 cells while normal cells. Besides, drug-loaded could be endocytosed Based these results, may serve carriers for therapeutic delivery.
sci-hub.st 参考文章(36)
Manuela Curcio, Barbara Blanco-Fernandez, Luis Diaz-Gomez, Angel Concheiro, Carmen Alvarez-Lorenzo, Hydrophobically Modified Keratin Vesicles for GSH-Responsive Intracellular Drug Release. Bioconjugate Chemistry. ,vol. 26, pp. 1900- 1907 ,(2015) , 10.1021/ACS.BIOCONJCHEM.5B00289
Weiping Jin, Wei Xu, Honghe Ge, Yan Li, Bin Li, Polyphenol–gelatin nanoparticles as reductant and stabilizer for one-step synthesis of gold nanoparticles and their interfacial behavior RSC Advances. ,vol. 5, pp. 26496- 26503 ,(2015) , 10.1039/C5RA02566D
Ru Cheng, Fang Feng, Fenghua Meng, Chao Deng, Jan Feijen, Zhiyuan Zhong, Glutathione-responsive nano-vehicles as a promising platform for targeted intracellular drug and gene delivery. Journal of Controlled Release. ,vol. 152, pp. 2- 12 ,(2011) , 10.1016/J.JCONREL.2011.01.030
F.H. Heijmen, J.S. du Pont, E. Middelkoop, R.W. Kreis, M.J. Hoekstra, Cross-linking of dermal sheep collagen with tannic acid. Biomaterials. ,vol. 18, pp. 749- 754 ,(1997) , 10.1016/S0142-9612(96)00202-5
S.T. Lim, G.P. Martin, D.J. Berry, M.B. Brown, Preparation and evaluation of the in vitro drug release properties and mucoadhesion of novel microspheres of hyaluronic acid and chitosan. Journal of Controlled Release. ,vol. 66, pp. 281- 292 ,(2000) , 10.1016/S0168-3659(99)00285-0
Vladimir Torchilin, Tumor delivery of macromolecular drugs based on the EPR effect Advanced Drug Delivery Reviews. ,vol. 63, pp. 131- 135 ,(2011) , 10.1016/J.ADDR.2010.03.011
Sitwat Lubna Khawar, Kenneth Watson, Graham L. Jones, High resolution one-dimensional electrophoretic separation and partial characterisation of human head hair proteins. Electrophoresis. ,vol. 16, pp. 110- 115 ,(1995) , 10.1002/ELPS.1150160120
Benjamin Thierry, Drug nanocarriers and functional nanoparticles: applications in cancer therapy. Current Drug Delivery. ,vol. 6, pp. 391- 403 ,(2009) , 10.2174/156720109789000474
Mikyung Shin, Ji Hyun Ryu, Joseph P. Park, Keumyeon Kim, Jae Wook Yang, Haeshin Lee, DNA/Tannic Acid Hybrid Gel Exhibiting Biodegradability, Extensibility, Tissue Adhesiveness, and Hemostatic Ability Advanced Functional Materials. ,vol. 25, pp. 1270- 1278 ,(2015) , 10.1002/ADFM.201403992
Kulbhushan Tikoo, Mukta Subhash Sane, Chanchal Gupta, Tannic acid ameliorates doxorubicin-induced cardiotoxicity and potentiates its anti-cancer activity: Potential role of tannins in cancer chemotherapy Toxicology and Applied Pharmacology. ,vol. 251, pp. 191- 200 ,(2011) , 10.1016/J.TAAP.2010.12.012