Secretion of IL-2 and IFN-γ, But Not IL-4, by Antigen-Specific T Cells Requires Extracellular ATP

摘要: Extracellular ATP and other nucleotides transmit signals to cells via surface-associated molecules whose binding sites face the extracellular milieu. Ecto-nucleoside triphosphate diphosphohydrolase is such an ATP-binding enzyme that expressed by activated lymphocytes. We have previously shown nonhydrolyzable analogs block lytic activity of NK CD8+ T as well their E-NTPDase activity. These results suggest hydrolysis may play a role in lymphocyte function. Here we report up-regulated within 15 min cell stimulation reversible irreversible inhibitors profoundly reduce secretion IL-2 IFN-γ, but not IL-4. TNF-α, IL-10, IL-5 production showed intermediate sensitivity these analogs. Depletion also inhibited IL-4, supporting interpretation required for some, all, cytokines. antagonists reduced transcription mRNA TCR-mediated intracellular calcium flux. plays essential signal transduction cascade expression certain cytokine genes.