Characterization of an antigen whose cell surface expression is induced by infection with Epstein-Barr virus.

摘要: Metabolically labeled monoclonal antibodies were used to measure the number of determinants per cell for an Epstein-Barr virus (EBV) surface antigen (EBVCS) (C. Kintner and B. Sugden, Nature [London] 294:458-460, 1981) which is expressed on EBV-transformed cells. The antigenic present approximately 5 X 10(5) times in vitro-transformed cell. Immunoprecipitation followed by electrophoresis polyacrylamide gels containing sodium dodecyl sulfate indicated that four independent EBVCS recognized a protein 47,000 daltons. identification isolated from cells grown tunicamycin demonstrated when without this drug was glycosylated. Finally, preclearing experiments with or HLA (class I products human major histocompatibility locus) beta 2-microglobulin not type 1 antigen.