Altered T cell activation and development in transgenic mice expressing the HIV-1 nef gene.

摘要: The nef gene, which encodes related cytoplasmic proteins in both human (HIV) and simian (SIV) immunodeficiency viruses is dispensable for viral replication vitro. In contrast, vivo experiments have revealed that SIV required efficient development of AIDS infected rhesus monkeys, thus indicating plays an essential role the natural infection. We show expression Nef protein from HIV-1 NL43 isolate transgenic mice perturbs CD4+ T cells thymus elicits depletion peripheral cells. Thymic expressing altered activation responses. HxB3 does not overt effect on when expressed animals. differential effects two alleles correlate with down-regulation CD4 antigen thymic interactions were reproduced a transient assay CEM Down-regulation by murine indicates relevant are conserved these systems suggests consequences host cell function can be analyzed system. Our observations suggest nef-elicited perturbations signalling play important life cycle vivo, perhaps resulting elimination