A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis.
作者： Xun Wang , Xia Cai , Hongdan Ma , Wen Yin , Li Zhu
DOI: 10.1038/S42003-019-0414-6
关键词: Transporter 、 Transcription (biology) 、 Operon 、 Intrinsic termination 、 Riboswitch 、 Cell biology 、 Gene 、 Untranslated region 、 Intracellular 、 Chemistry
摘要: The intracellular K+ level in bacteria is strictly controlled by uptake and efflux systems. Among these, KdpFABC a high-affinity transporter system that generally activated the KdpDE two-component response to limitation stress. However, regulatory mechanism remains obscure lacking kdpDE genes. Here we report transcription of kdpFABC operon distinctively regulated cyclic diadenylate monophosphate (c-di-AMP) riboswitch located at 5'-untranslated region kdp transcript, binding c-di-AMP promotes its intrinsic termination blocks transcription. Further, concentration was found decrease under stress, leading transcriptional read-through over terminator allow expression. This element predominantly Bacillus cereus group correlate well with homeostasis affects variety crucial cellular functions.
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