Analysis of plasmid-mediated quinolone and oxyimino-cephalosporin resistance mechanisms in Uruguayan Salmonella enterica isolates from 2011-2013.

摘要: This study characterised the mechanisms of fluoroquinolone and oxyimino-cephalosporin resistance in human Salmonella enterica isolates Uruguay. were collected from 2011-2013 selected based on non-susceptibility to ciprofloxacin and/or oxyimino-cephalosporins. The disk diffusion assay was performed for various antibiotics, minimum inhibitory concentration (MIC) determined following CLSI guidelines. Genetic relatedness PulseNet protocols. Extended-spectrum β-lactamases, ampC alleles plasmid-mediated quinolone by PCR sequencing. Plasmid analyses carried out conjugation or transformation assays, plasmid-encoded genes identified PCR. Mutations resistance-determining region gyrases sought Among 579 isolates, 105 (18.4%) ciprofloxacin-non-susceptible (CIP-NS) 9 (1.6%) oxyimino-cephalosporin-resistant 2 (0.3%) resistant both antibiotic families detected. Thirteen qnrB (twelve qnrB19 one qnrB2), four blaCTX-M-8, two blaCTX-M-14, blaSHV-2 three blaCMY-2-like genes. No correlation found between mutations MICs. Several co-circulating clones S. ssp. serovar Typhimurium detected; conversely, Enteritidis corresponded mainly a single circulating clone. Nine (75%) twelve CIP-NS extraintestinal shared same pulsotype with intestinal isolates. During period, frequency increased, albeit MICs 0.125-0.5mg/L. Detection quinolone-resistant recovered samples highlights significance epidemiological surveillance susceptibility every isolate.