Cas6 processes tight and relaxed repeat RNA via multiple mechanisms: A hypothesis.
作者： Jana Sefcikova , Mitchell Roth , Ge Yu , Hong Li
关键词: RNA Conformation 、 Biology 、 Signal recognition particle RNA 、 Non-coding RNA 、 Intron 、 RNA editing 、 Genetics 、 RNA silencing 、 Riboswitch 、 RNA 、 Computational biology
摘要: RNA molecules are flexible yet foldable. Proteins must cope with this structural duality when forming biologically active complexes RNA. Recent studies of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs)-mediated immunity illustrate some remarkable mechanisms which proteins interact Currently known structures CRISPR-Cas6 endoribonucleases bound suggest a conserved protein recognition mechanism mediated by stem-loops. However, survey CRISPR reveals that many repeats either lack productive stem-loop (Relaxed) or possess stable but inhibitory (Tight), raises question how enzyme processes structurally diverse In reviewing recent literature, we propose bivalent trapping and an unwinding for to Relaxed Tight repeat RNA, respectively. Both aim create identical conformation at cleavage site accurate processing.
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