Treg-promoted New Bone Formation Through Suppressing TH17 by Secreting Interleukin-10 in Ankylosing Spondylitis.

摘要: STUDY DESIGN Retrospective single-center study. OBJECTIVE We want to know whether interleukin (IL)-10-secreting regulatory T cells (Treg) promote the new bone formation (NBF) through suppressing TH17 in ankylosing spondylitis (AS). SUMMARY OF BACKGROUND DATA NBF AS is unknown. Since there are balances of remodeling human body and proinflammatory helper promoted resorption. METHODS Eighteen patients with or without (both nine cases) healthy individuals were selected demographic data, Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), MRI sacroiliitis score (MRISIS), computer tomography (CTSIS) recorded. Removed hip ligament tissue lesions after arthroplasty was collected lymphocytes peripheral blood mononuclear prepared. Second, pathological section hematoxylin-eosin stain analyzed flow cytometry quantitative polymerase chain reaction analyses carried out detect levels TH17, Treg, IL-10, nuclear factor (NF)-κB, relevance between them. The effect Treg on further by using Transwell coculturing. RESULTS Compared NBF, had significantly higher CTSIS complications (P < 0.05 0.01, respectively), but lower BASDAI (3.0 ± 0.4) MRISIS (3.3 ± 0.8) 0.01 0.05, respectively) no acute inflammation HE for joint. donors, ratio TH17/Treg patient P 0.01) analysis (FCA). Furthermore, decreased indirectly coculturing FCA 0.01). Finally, IL-10 mRNA expression 0.01), NF-κB 0.05) than donors. Only correlated (r = -0.93, CONCLUSION Treg-induced secreting IL10 declining indicated development NBF. This important not only screening also control immune therapy. LEVEL EVIDENCE N/A.