Ribonucleic acids from yeast which contain a fifth nucleotide.
作者： Frank F. Davis , Frank Worthington Allen
DOI: 10.1016/S0021-9258(18)70770-9
关键词: Saccharomyces cerevisiae 、 Ribonuclease 、 Sodium 、 Biology 、 Biochemistry 、 Nucleotide 、 Yeast 、 RNA analysis 、 Yield (chemistry)
摘要: All recent methods for the isolation of ribonucleic acids from yeast yield only 20 to 70 per cent that can be assumed present on basis quantitative analyses. There have been no studies regarding nature are lost during isolation, but it has tacitly those which same properties as isolated. In procedure Crestfield, Smith, and Allen (I), 60 bakers’ is isolated by a final precipitation 1 M solution sodium chloride at 0”. 10 total soluble in chloride, together with certain proteins deoxyribonucleic acids. The communication deals purification, characterization also describes fifth nucleotide component became evident studying action ribonuclease these Preparation Fractions
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sci-hub.st 参考文章(14)
R. Markham, J. D. Smith, Chromatographic studies of nucleic acids. 1. A technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substances Biochemical Journal. ,vol. 45, pp. 294- 298 ,(1949) , 10.1042/BJ0450294
Arthur M. Crestfield, Kendric C. Smith, Frank Worthington Allen, The preparation and characterization of ribonucleic acids from yeast. Journal of Biological Chemistry. ,vol. 216, pp. 185- 193 ,(1955) , 10.1016/S0021-9258(19)52295-5
Boris. Magasanik, Ernst. Vischer, Ruth. Doniger, David. Elson, Erwin. Chargaff, The separation and estimation of ribonucleotides in minute quantities. Journal of Biological Chemistry. ,vol. 186, pp. 37- 50 ,(1950) , 10.1016/S0021-9258(18)56284-0
A. M. Crestfield, F. W. Allen, Resolution of Ribonucleotides by Zone Electrophoresis Analytical Chemistry. ,vol. 27, pp. 424- 425 ,(1955) , 10.1021/AC60099A029
Waldo E. Cohn, The Anion-Exchange Separation of Ribonucleotides Journal of the American Chemical Society. ,vol. 72, pp. 1471- 1478 ,(1950) , 10.1021/JA01160A012
S. M. PARTRIDGE, Aniline Hydrogen Phthalate as a Spraying Reagent for Chromatography of Sugars Nature. ,vol. 164, pp. 443- 443 ,(1949) , 10.1038/164443A0
Waldo E. Cohn, HETEROGENEITY IN PYRIMIDINE NUCLEOTIDES Journal of the American Chemical Society. ,vol. 72, pp. 2811- 2812 ,(1950) , 10.1021/JA01162A546
WALDO E. COHN, ELLIOT VOLKIN, Nucleoside-5′-Phosphates from Ribonucleic Acid Nature. ,vol. 167, pp. 483- 484 ,(1951) , 10.1038/167483A0
R. Markham, J. D. Smith, The structure of ribonucleic acids. 1. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysis Biochemical Journal. ,vol. 52, pp. 552- 557 ,(1952) , 10.1042/BJ0520552
Kendric C. Smith, Frank Worthington Allen, The Liberation of Polynucleotides by the Alkaline Hydrolysis of Ribonucleic Acid from Yeast Journal of the American Chemical Society. ,vol. 75, pp. 2131- 2133 ,(1953) , 10.1021/JA01105A031