Transcriptome analysis of growth variation in early juvenile stage of sandfish Holothuria scabra

作者: June Feliciano F. Ordoñez , Gihanna Gaye ST. Galindez , Rachel Ravago-Gotanco

DOI: 10.1101/2020.09.01.273102

关键词: HatcheryMaricultureEvolutionary biologyDe novo transcriptome assemblyJuvenileSea cucumberBiologyExtracellular matrix organizationTranscriptomeHolothuria scabra

摘要: Abstract The sandfish Holothuria scabra is a high-value tropical sea cucumber species representing major mariculture prospect across the Indo-Pacific. Advancements in culture technology, rearing, and processing present options for augmenting capture production, stock restoration, sustainable livelihood activities from hatchery-produced sandfish. Further improvements production may be gained application of genomic technologies to improve performance traits such as growth. In this study, we performed de novo transcriptome assembly characterization fast- slow-growing juvenile H. three Philippine populations. Analyses revealed 66 unigenes that were consistently differentially regulated fast-growing found associated with immune response metabolism. Further, identified microsatellite single nucleotide polymorphism markers potentially fast These findings provide insight on potential determinants underlying growth regulation early which will useful further functional studies. Highlights study explores basis variation by examining gene expression profiles stages hatchery populations using RNA-seq. Sixty-six related are several biological molecular processes, including carbohydrate binding, extracellular matrix organization, fatty-acid metabolism, metabolite solute transport. A large number category-associated SNP have been identified.

参考文章(118)
Kalpna Gupta, Pankaj Gupta, Robert Wild, Sundaram Ramakrishnan, Robert P. Hebbel, Binding and displacement of vascular endothelial growth factor (VEGF) by thrombospondin: effect on human microvascular endothelial cell proliferation and angiogenesis. Angiogenesis. ,vol. 3, pp. 147- 158 ,(1999) , 10.1023/A:1009018702832
David J. DeRosier, Lewis G. Tilney, The form and function of actin. A product of its unique design. Cell and muscle motility. ,vol. 5, pp. 139- 169 ,(1984) , 10.1007/978-1-4684-4592-3_3
A. R. P. Paterson, J. Frank Henderson, Nucleotide Metabolism: An Introduction ,(1973)
Colin Thorpe, Jujng‐Ja P. Kim, Structure and mechanism of action of the acyl-CoA dehydrogenases. The FASEB Journal. ,vol. 9, pp. 718- 725 ,(1995) , 10.1096/FASEBJ.9.9.7601336
Geraldo S Magalhães, M Lopes-Ferreira, IdLM Junqueira-de-Azevedo, PJ Spencer, MS Araújo, Fernanda Calheta Vieira Portaro, L Ma, RH Valente, L Juliano, JW Fox, PL Ho, Ana Maria Moura-da-Silva, None, Natterins, a new class of proteins with kininogenase activity characterized from Thalassophryne nattereri fish venom. Biochimie. ,vol. 87, pp. 687- 699 ,(2005) , 10.1016/J.BIOCHI.2005.03.016
T. Thiel, W. Michalek, R. Varshney, A. Graner, Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.). Theoretical and Applied Genetics. ,vol. 106, pp. 411- 422 ,(2003) , 10.1007/S00122-002-1031-0
Alan Peterkofsky, The mechanism of action of histidase: amino-enzyme formation and partial reactions. Journal of Biological Chemistry. ,vol. 237, pp. 787- 795 ,(1962) , 10.1016/S0021-9258(18)60373-4
N.N.S. Agudo, Sandfish hatchery techniques Research Papers in Economics. ,(2006)
Susan Hutson, Structure and function of branched chain aminotransferases. Progress in Nucleic Acid Research and Molecular Biology. ,vol. 70, pp. 175- 206 ,(2001) , 10.1016/S0079-6603(01)70017-7