Antioxidant activity of phenolic extracts from different cultivars of Italian onion (Allium cepa) and relative human immune cell proliferative induction
作者： Antonella Lisanti , Vincenzo Formica , Federica Ianni , Barbara Albertini , Maura Marinozzi
DOI: 10.3109/13880209.2015.1080733
关键词: CD8 、 Food science 、 Liliaceae 、 Traditional medicine 、 Biology 、 Peripheral blood mononuclear cell 、 Allium 、 ABTS 、 Cytotoxic T cell 、 DPPH 、 Immune system
摘要: AbstractContext: The total antioxidant activity (TAC) may vary considerably between onion cultivars. Immunological effects of phenolic compounds are still underestimated.Objective: objective this study is to determine the phenol content (TPC) and relative TAC three Allium cepa L. (Liliaceae) cultivars cultivated in Cannara (Italy): Rossa di Toscana, Borettana Rovato, Dorata Parma, evaluate extracts ability induce human immune cell proliferation.Materials methods: TPC was determined by Folin–Ciocalteu method, with FRAP, TEAC/ABTS, DPPH methods. Peripheral blood mononuclear cells from healthy donors were incubated for 24 h at 37 °C 1 ng/mL extract PBS, immunostained, then analyzed 4-color flow cytometry phenotypic characterization T helper (CD4+ cells), cytotoxic lymphocytes (CD8+ regulatory (CD25high CD4+ natural killer cells/monocytes (CD16+ cells)....
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