Cross-reactivity of metallothioneins from different origins with rabbit anti-rat hepatic metallothionein antibody
作者： Chiharu Tohyama , Zahir A. Shaikh
DOI: 10.1016/0006-291X(78)91669-8
关键词: Cross-reactivity 、 Metallothionein 、 Disc electrophoresis 、 Antibody 、 Precipitin 、 Molecular biology 、 Antiserum 、 Rabbit (nuclear engineering) 、 Ammonium sulfate 、 Biology
摘要: Abstract Rat hepatic Cd-metallothionein was purified and isolated into its two components, metallothionein 1 2, by disc electrophoresis. Antibodies to 2 were generated in rabbits. The antiserum reacted with the protein formed a single precipitin band on double diffusion plate. By ammonium sulfate precipitations, it found that cross-reacted rat 1. Cross-reactivity of also observed for components renal Cd-metallothionein, rabbit human metallothionein.
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