Viability of forty-two neoplasms after long-term storage in liquid nitrogen at −195 °C
作者： Isidore Wodinsky , Kathleen F. Meaney , C.J. Kensler
DOI: 10.1016/0011-2240(71)90108-8
关键词: Population based 、 Microbiology 、 Liquid nitrogen 、 Cell 、 In vivo 、 Inoculation 、 Cell kinetics 、 Pathology 、 Bioassay 、 Biology 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Forty-two neoplasms stored at −195 °C for periods up to 6 years have remained transplantable and lethal their inoculated hosts. A bioassay which estimates the surviving cell population based on kinetics of exponentially growing in vivo demonstrated an initial loss viable cells due freezing procedures no further losses storage. The response 14 drug-induced resistant chemotherapeutic agents has unaltered after long-term storage.
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