Unstructured Modeling of a Synthetic Microbial Consortium for Consolidated Production of Ethanol

摘要: Abstract A defined mixed culture of specialized microbes that exploits the native capabilities each member species is a promising alternative to use single engineered microbe for cellulosic biofuels production. We explored such synthetic consortium couples high cellulolytic activity filamentous fungus Trichoderma reesei with ability yeasts Saccharomyces cerevisiae and Scheffersomyces stipitis ferment hexose pentose sugars ethanol. Consortium stability was demonstrated by culturing three on mixture cellulose xylan. As first step towards understanding manipulating this consortium, we developed simple dynamic model unstructured descriptions enzyme synthesis, hemicellulose degradation, sugar uptake, cell growth, ethanol The batch contained 10 ordinary differential equations parameters obtained from literature experiment extent possible. used predict initial concentration type maximized productivity fixed total inoculum concentration. simulated ratio in feedstock varied determine effects optimal productivity.