Impaired hepatic mitochondrial oxidation using the 13C-methionine breath test in patients with macrovesicular steatosis and patients with cirrhosis.

摘要: BACKGROUND In cirrhosis, hepatic mitochondriae exhibit morphological and functional abnormalities. human steatosis, ultrastructural changes are reported in the absence of ethanol. Routine evaluation mitochondrial function is difficult. We used a breath test to explore oxidation vivo. MATERIAL/METHODS The 13C-methionine was performed healthy subjects (n=15), patients with cirrhosis (n=25), biopsy-proven severe (> 40% involved hepatocytes), non-alcoholic macrovesicular steatosis (n=18). After oral administration (200 mg), decarboxylation measured by [13C]-C02 enrichment, expressed as dose/h delta over base (DOB), for 1 hour, isotope ratio infrared spectroscopy. Results were normalized against BMI. RESULTS At 60 minutes, had reduced exhalation 13C02 compared (dose/h: -47%, 18.8+/-12 vs 36+/-6.1; DOB: -52%, 40.4+/-32 85+/-20, p<0.05). Cirrhotics even lower values -60%, 7.5+/-3.4 18.8+/-12, correlated (r=0.68) aminopyrine values, microsomal test, inversely (r=-0.48) Child-Pugh score. CONCLUSIONS Hepatic reflected impaired both pure non alcohol-related mixed etiologies. This non- invasive can be monitor