Probing the size limit for nanomedicine penetration into Burkholderia multivorans and Pseudomonas aeruginosa biofilms

摘要: Encapsulation of antibiotics into nanoparticles is a potential strategy to eradicate biofilms. To allow further optimization nanomedicines for biofilm eradication, the influence nanoparticle size on penetration dense clusters needs be investigated. In present study, with diameters ranging from 40 550 nm two biofilms, Burkholderia multivorans LMG 18825 and Pseudomonas aeruginosa 27622, was evaluated using confocal microscopy. Through image analysis, percentage particles able penetrate calculated. The cut off optimal located around 100-130 both mesh matrix channels in between bacteria are factors which likely play role exclusion larger particles. For B. multivorans, sharp drop seen than 130 while P. aeruginosa, more gradual decrease could observed. overall slightly lower multivorans. Based these results, it concluded that nanocarriers about 100 smaller good candidates improve treatment chronic pulmonary biofilms CF patients. Furthermore, microscopy method demonstrated here useful tool assess clusters. Such information important optimize nanomedicine formulations infections.