Spectrofluorometric and spectrophotometric investigations of cyclophosphazene-DNA complexes
作者： Jean-Luc Butour , Jean-François Labarre , François Sournies
DOI: 10.1016/0022-2860(80)85179-9
关键词: Uv spectrophotometry 、 Fluorescence 、 Ethidium bromide 、 DNA 、 Photochemistry 、 Stereochemistry 、 Chemistry
摘要: Abstract A series of cyclophosphazene—DNA matrices is investigated by UV spectrophotometry and spectrofluorometry (using EtdBr (ethidium bromide) as a probe) in order to gain information about the structural modifications DNA due different possible modes interaction thus attempt explain origin their antitumour properties. There no direct relationship between activity fluorescence decrease (and/or hyper-bathochromic shift, when observed) suggesting that targets other than must be looked at real mode action cyclophosphazenes agents.
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sci-hub.se 参考文章(14)
Jean-Bernard Le Pecq, Use of Ethidium Bromide for Separation and Determination of Nucleic Acids of Various Conformational Forms and Measurement of Their Associated Enzymes Methods of Biochemical Analysis. ,vol. 20, pp. 41- 86 ,(1971) , 10.1002/9780470110393.CH2
J.-B. Lepecq, C. Paoletti, A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. Journal of Molecular Biology. ,vol. 27, pp. 87- 106 ,(1967) , 10.1016/0022-2836(67)90353-1
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Bruce N. Ames, Joyce McCann, Edith Yamasaki, Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity test Mutation Research. ,vol. 31, pp. 347- 364 ,(1975) , 10.1016/0165-1161(75)90046-1
M.J. Waring, Complex formation between ethidium bromide and nucleic acids. Journal of Molecular Biology. ,vol. 13, pp. 269- 282 ,(1965) , 10.1016/S0022-2836(65)80096-1
George Scatchard, The Attractions of Proteins for Small Molecules and Ions Annals of the New York Academy of Sciences. ,vol. 51, pp. 660- 672 ,(1949) , 10.1111/J.1749-6632.1949.TB27297.X
R. Keat, R. A. Shaw, Phosphorus–nitrogen compounds. Part XX. The reaction of piperidine with hexachlorocyclotriphosphazatriene; the replacement pattern and the structure of the products; the “cis-effect” Journal of The Chemical Society A: Inorganic, Physical, Theoretical. pp. 908- 913 ,(1966) , 10.1039/J19660000908
J. Galy, R. Enjalbert, J.-F. Labarre, The first example of a cyclophosphazene `anticlathrate structure': geometry of 2,2,4,4,6,6-hexa(1-aziridinyl)cyclotri(phosphazene), N3P3az6, from an X-ray investigation of the complex N3P3az6.3CCl4 Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry. ,vol. 36, pp. 392- 397 ,(1980) , 10.1107/S0567740880003330
Rudi Ratz, Ehrenfried Kober, Christoph Grundmann, Gerhard Ottmann, Syntheses and Reactions of 2,2,4,4,6,6-Hexakis(1-aziridinyl)cyclotriphosphaza-1,3,5-triene and Related Compounds Inorganic Chemistry. ,vol. 3, pp. 757- 761 ,(1964) , 10.1021/IC50015A033
Jan Olov Bovin, Jean Galy, Jean-François Labarre, François Sournies, Cyclophosphazenes as novel potential antitumor agents: x-ray crystal structure of the octapyrrolidinocyclotetraphosphazene, N4P4(NC4 H8)8 Journal of Molecular Structure. ,vol. 49, pp. 421- 423 ,(1978) , 10.1016/0022-2860(78)87282-2