3D-Printed Enzyme-Embedded Plastics.

摘要: A simple and environmentally friendly approach toward the thermoplastic processing of rapidly degradable plastic-enzyme composites using three-dimensional (3D) printing techniques is described. Polycaprolactone/Amano lipase (PCL/AL) composite films (10 mm × 10 mm; height [h] = ∼400 μm) with an AL loading 0.1, 1.0, 5.0% were prepared via 3D that entail direct mixing in solid state thermal layer-by-layer extrusion. It was found can tolerate situ temperatures up to 130 °C solid-state for 60 min without loss enzymatic activity. The degraded phosphate buffer (8 mg/mL, buffer) 7 days at 37 resulting average percent total weight (WLavg %) be 5.2, 92.9, 100%, films, respectively. degradation rates PCL/AL faster than applied externally buffer. Thicker 1.0% h ∼500 also over a day period examine how occurs time 3.0, 18.1, 36.4, 46.4, 70.2% 1, 2, 3, 4, 7, Differential scanning calorimetry (DSC) analysis shows film's crystallinity (Dxtal%) increases Dxtal% 46.5 0 53.1% 7. Scanning electron microscopy (SEM) film erosion begins surface water penetrate interior pores activating enzymes embedded film. Controlled release experiments utilizing dye-loaded PCL/AL/dye (AL 1.0%; dye 0.1%) bulk released by fourth day. multimaterial objects containing AL-resistant polylactic acid (PLA) printed demonstrate application this material on more complex structures.