Mode of PGE/sub 2/ -mediated T lymphocyte inactivation at the fetomaternal interface

摘要: The authors have shown that first trimester human decidual cells (DC) suppress lymphocyte alloreactivity (MLR and CML) by secreting PGE/sub 2/. Present study examined whether this resulted from (1) a blockade in the generation of IL-2 receptors on responder cells, (2) an inhibition production (3) interference with interaction between its receptors, or (4) CTL lytic function. (Tac Ag) were measured radioimmunoassay radioautography 4d MLC lymphocytes cultured for 0.5 - 4 d con A +/- various doses irradiated DC (+/-10/sup -5/M indomethacin) 2/ (2.3 x 10/sup -5/ -6/M). produced above set up was assayed against recombinant (rIL-2) standard measuring proliferative response (/sup 3/HTdR uptake) dependent clone CTLL-2. Effects presence (+/- CTLL-2 rIL-2 noted. Finally effects same also noted killer function generated 7d MLC, /sup 51/Cr releasemore » assay. Results revealed caused both receptor development production, being reversible indomethacin. They did not interfere proliferation CTL.« less