Improved superfusion technique for rapid cooling or heating of cultured cells under patch-clamp conditions.

作者: Ivan Dittert , Jan Benedikt , Ladislav Vyklický , Katharina Zimmermann , Peter W. Reeh

DOI: 10.1016/J.JNEUMETH.2005.07.005

关键词: Temperature controlJoule heatingAnalytical chemistryMicromanipulatorBody orificeChemistryHeating elementOptoelectronicsThermoelectric coolingThermocoupleCapillary action

摘要: … current source for resistive heating. The Peltier device and the heating element are electrically connected to the headstage probe … activity in isolated DRG neurons under current clamp. …

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