Avaliação dos parâmetros espermáticos e caracterização do perfil bioquímico do plasma seminal de cães (Canis familiaris – LINNAEUS, 1758) com hiperplasia prostática benigna, tratados com toxina botulínica A
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摘要: xvii 1. INTRODUCAO 1 2. REVISAO BIBLIOGRAFICA 2 2.1 Anatomia, histologia e fisiologia prostatica 2.2 Hiperplasia benigna (HPB) 4 2.3 Neurotoxina botulinica tipo A 13 2.4 Metodos de avaliacao da fertilidade 24 3. MATERIAL E METODOS 31 3.1 Animais grupos experimentais 3.2 Toxina 32 3.2.1 – Diluicao 3.2.2 Injecao toxina do solucao fisiologica NaCl 0,9% 3.3 Colheita semen 34 3.4 Analises fisicas morfologicas 35 3.4.1 macroscopicas 3.4.2 microscopicas 36 3.4.2.1 Motilidade, vigor concentracao espermatica .. 3.4.2.2 Morfologia 3.4.2.3 Avaliacao das membrana plasmatica 37 3.4.2.3.1 Teste hiposmotico 3.4.2.3.2 Coloracao por sondas Fluorescentes
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