Induction of NK cell activity against fresh human leukemia in culture with interleukin 2.

摘要: Natural killer (NK) cells have been implicated in defense against malignancies, especially leukemia. Because patients with leukemia and preleukemic disorders manifest low NK activity, it is possible that cell impairment may contribute to leukemogenesis. In view of this possibility, was important characterize the defect leukemic design new approaches for its correction. Analysis mechanism demonstrated were impaired their tumor-binding lytic activity did not display ability recycle or produce cytotoxic factor. However, deficient could be corrected by culture peripheral blood effector IL 2. 2-activated manifested restoration all measured parameters mechanism, as exemplified normalized well rate lysis recycle. Importantly, such vitro stimulated displayed reactivity fresh autologous allogeneic origin. Another interesting observation from these studies also induced bone marrow, a tissue very frequency cells. It note cultured represent stationary population, but proliferated quite actively (doubling time 3 6 days) at least 5 wk. Characterization generated indicated large granular lymphocyte morphology CD16 Leu-19 surface phenotype. Our data demonstrate permanent phenomenon, can reversed 2, fully maintained expanded vitro. Thus, reasonable suggest adoptive transfer promising therapy treatment