Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages

摘要: Tuberculosis (TB) remains a significant global health concern and its diagnosis is challenging due to the limitations in specificity sensitivity of current diagnostic tests. Exosomes are bioactive 30–100 nm vesicles produced by most cell types found almost all human body fluids. Exosomal microRNAs (miRNAs) can transfer biological information between cells tissues may act as potential biomarkers many diseases. In this pilot study, we assessed miRNA profile exosomes released from monocyte-derived macrophages upon infection with Mycobacterium bovis Bacillus Calmette–Guerin (BCG). Human monocytes were obtained peripheral blood three healthy subjects driven macrophage (MDM) phenotype using standard protocols. MDMs infected BCG or left uninfected control. 72 h post-infection, collected culture medium, RNA was isolated RNA-seq performed. The raw reads filtered eliminate adaptor primer sequences run against mature available miRBase. MicroRNAs identified an E value <0.01. network analysis performed DIANA tool, miRDB functional KEGG pathway analysis. Infection leads release several exosomal miRNAs. These included miR-1224, -1293, -425, -4467, -4732, -484, -5094, -6848-6849, -4488 -96 which predicted target metabolism energy production-related pathways. This study provides evidence for specific miRNAs BCG-infected MDMs. reflect host-pathogen interaction subversion host metabolic processes following infection.