Molecular analysis of zyg-11, a maternal-effect gene required for early embryogenesis of Caenorhabditis elegans.

摘要: The product of the maternally acting gene zyg-11 is required for early embryogenesis Caenorhabditis elegans. One-cell embryos that lack a functional exhibit an arrest meiosis at metaphase II, delay in formation pronuclei, unusually vigorous movements cytoplasm, multiple incorrect segregation P granules, and placement first cleavage furrow. We have isolated sequenced molecular clone zyg-11, shown microinjection cloned DNA can rescue mutations. A transcriptional analysis shows transcription not limited to female germ-line, despite strict maternal-effect phenotype