Error Production and Error Propagation During PCR

摘要: Amplification factors achieved during routine applications of PCR are in the range 106–108. Given these high amplification it is important to know rate error production process. The frequency base substitutions Taq DNA polymerase a single round replication has been determined be order 1 × 10-4 [4]. rather as compared other polymerases due lack an exonuclease proofreading activity this enzyme. In present study we have and propagation errors function number cycles.